Research Article

Streptomyces misionensis PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates

Table 5

Effect of metal ions on endoglucanase activity. Enzyme was produced by S. misionensis PESB-25 grown on 1.0% (w/v) SCB and 1.2% (w/v) CSL.

IonaRelative activity
(%)*
Endoglucanase activity
(U·mL−1)

Control (no addition)100.01.72
NaCl133.2 ± 2.02.23
CuSO4140.6 ± 0.32.30
MgSO4126.6 ± 0.92.18
CoSO4161.2 ± 0.62.73
MnSO4201.5 ± 0.13.48
FeSO4131.1 ± 0.12.34
CaCl2137.6 ± 2.32.25
KCl125.3 ± 4.92.17
BaCl2109.3 ± 0.91.97

The final concentration in the reaction mixture was 2 mM.
Values are based on Mean ± SD of 3 individual observations.