Cloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa

Table 2

Equilibrium dissociation constants for the binding of GDP to P. aeruginosa EF-Tu.

EF-Tu^1free; (×10^−3 μM)	GDPfree; (×10^−1 μM)	EF-Tu · GDP; (×10^−3 μM)	EF-Ts; (×10^−3 μM)	Temperature; (°C)	𝐾GDP; (nM)
1.3	1.2	4.5	0.5	37	34
1.2	1.2	4.6	0.5	23	30
1.5	1.2	4.2	0.5	0	43
1.5	1.2	2.5	0	37	72
1.0	1.2	3.2	0	23	38
1.3	1.2	2.7	0	0	58

1EF-Tu_free and GDP_free as described under Section 3.6.