Cloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa

Table 3

Equilibrium dissociation constants for the binding of GTP to P. aeruginosa EF-Tu.

EF-Tufree; (×10^−3 μM)	GTPfree; (×10^−1 μM)	EF-Tu · GTP; (×10^−3 μM)	EF-Ts; (×10^−3 μM)	Temperature; (°C)	𝐾GTP; (nM)
4.2	1.2	2.6	0.5	37	190
3.5	1.2	3.3	0.5	23	125
3.9	1.2	2.9	0.5	0	156
4.1	1.2	2.7	0	37	175
3.5	1.2	3.3	0	23	123
3.8	1.2	2.9	0	0	150