Figure 1: (a) Clinical profile of EAE. Female C57BL/6 mice ( ) were immunized with MOG 35–55 and scored daily using a previously reported 5-point scale of ascending paralysis [32, 33]. Data represent mean and standard error of the mean (SEM) generated from multiple animals in one experiment. One representative experiment of three is shown. (b) RSA59 replication in the brains of mice. C57BL/6 mice were infected with 20,000 PFU of RSA59 by intracranial inoculation. Mice were sacrificed at days 1, 3, 5, and 7 after inoculation, and viral titers were determined by plaque assay as in prior studies [36, 39]. The data represent the means (and standard deviations) of the titers from five mice. Titers are expressed as log10 PFU per gram of tissue.