Different Mechanisms of Inflammation Induced in Virus and Autoimmune-Mediated Models of Multiple Sclerosis in C57BL6 Mice
Figure 4
Spinal cord inflammation and demyelination in RSA59 infection. Representative cross-sections of spinal cords (5 μm thick) of virally infected mice at different days after inoculation were stained with LFB (for myelin) and immunohistochemically for CD45 (LCA), CD3 (T cells), and Iba1 (macrophages/microglia). ((a), (c), (e), (g)) Day 7 after inoculation (peak of inflammation). ((b), (d), (f), (h)) Day 30 after inoculation (late phase). ((a), (b)) LCA stain. ((c), (d)) Luxol fast blue. ((e), (f)) CD3. ((g), (h)) Iba1. At day 7 after inoculation, both gray and white matter are infiltrated by LCA positive cells, whereas by day 30, LCA immunoreactivity is predominately localized to the white matter. In contrast, there are few CD3 positive cells at both early and late phases. Iba1 immunoreactivity increases and becomes progressively localized to demyelinating plaques. Inset in (e) shows higher magnification of rare CD3 immunoreactive lymphocytes. LFB stains demonstrate increasing numbers and sizes of demyelinating plaques over time. Arrows mark locations of demyelinating plaques.
