Assessing Competence of Broccoli Consumption on Inflammatory and Antioxidant Pathways in Restraint-Induced Models: Estimation in Rat Hippocampus and Prefrontal Cortex

Figure 3

Western blot analysis to measure the expression of the major proteins of the antioxidant Nrf-2 pathway, nuclear Nrf-2, HO-1, and NQO-1 in the hippocampus and prefrontal cortex tissues derived after 6 hours of restraint-induced stress in experimental groups. (a) Immunoblot bands of NQO-1, HO-1, and nuclear Nrf-2, as well as their relevant β-actin and lamin B2. ((b)–(d)) The densities of corresponding bands were measured and their respective ratios to β-actin and lamin B2 and were calculated and represented as arbitrary units on the graphs for each experimental group (𝑛=6). Bars indicate the mean ± SEM; one-way ANOVA; *𝑃<0.05; **𝑃<0.01; ***𝑃<0.001.

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