Assessing Competence of Broccoli Consumption on Inflammatory and Antioxidant Pathways in Restraint-Induced Models: Estimation in Rat Hippocampus and Prefrontal Cortex

Figure 5

Western blot analysis to measure the expression of the important proteins of the caspase-dependent and caspase-independent apoptotic cell death, cleaved caspase-3 and AIF, respectively, in the hippocampus and prefrontal cortex tissues derived after 6 hours of restraint-induced stress in experimental groups. (a) Immunoblot bands of cleaved caspase-3 and nuclear AIF, as well as their relevant β-actin and lamin B2 bands. Changes in the expression of 17 kDa fragment of cleaved caspase-3 are considered. ((b) and (c)) The densities of corresponding bands were measured and the ratios to β-actin and lamin B2 were calculated and represented as arbitrary units on the graph for each experimental group (𝑛=6). Bars indicate the mean ± SEM; one-way ANOVA; *𝑃<0.05; **𝑃<0.01; ***𝑃<0.001.

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