Biochemical Characterization and Pharmacological Properties of New Basic PLA₂ BrTX-I Isolated from Bothrops roedingeri (Roedinger's Lancehead) Mertens, 1942, Snake Venom

Table 1

Measured molecular masses and deduced amino acid sequences obtained by ESI-MS/MS based on the alkylated tryptic peptides of BrTX-I. The peptides were separated by RP-HPLC and sequenced by mass spectrometry. C = alkylated cysteine, lysine residues shown in bold were deduced on the cleavage and missed cleavage by trypsin. All molecular masses are reported as monoisotopic.

BrTX-I HPLC fraction	Measured mass (Da)	Amino acid sequence	Theoretical mass (Da)
1	1360.65	DL/IWQ/KWNK/QMI/LK/Q	1360.61
2	1404.67	DI/LTI/LVCGEDL/IPCK/Q	1404.64
3	1791.07	AAAVCFYENL/IGTYNK/QK/Q	1791.03
4	1120.28	YGCYCGWGGR	1020.25
5	616.79	L/ITGCPK/Q	616.75