Heavy Water Reduces GFP Expression in Prokaryotic Cell-Free Assays at the Translation Level While Stimulating Its Transcription
Figure 4
Schematics of the observed effect of D2O on transcription, translation, and GFP maturation in the cell-free protein synthesis system. Transcription efficiency was found to increase, whereas translation and maturation efficiencies were diminished. The observed overall effect of D2O on the GFP expression rate is negative (see text for details).
