Research Article

Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction

Figure 1

The expression profiles of germ cell-associated genes during EB formation from iPS cells. Real-time PCR was performed using cDNA from EB cultures maintained for 0, 4, 7, and 14 days (D0, D4, D7, and D14) to detect the relative mRNA expression of Dppa3, Stra8, Tex14, Dazl, Scp1, Scp3, Msy2, and Akap3. The mean normalized expression of each gene relative to that of Gapdh was shown.
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