Figure 7: Immunolocalization of insulin and Pdx-1. Pdx-1 (red) shows increased nuclear localization in (a) 1-month Mutants followed by (b) 6-month Mutants and (c) 12-month Mutants compared with their Leans and Controls among insulin (green) positive β-cells. The localization was almost similar between 12-month Mutants and (d) older age (24-month) Controls. RFU has been represented (e) amongst phenotypes. Statistical significance ( by ANOVA) has been represented compared to Control by an asterisk (*) and compared to younger age by ($).