Research Article

The Impact of Aspergillus fumigatus Viability and Sensitization to Its Allergens on the Murine Allergic Asthma Phenotype

Figure 5

Effect of inhaled dead or live A. fumigatus conidia on airway remodeling in non-sensitized murine host. Periodic acid-Schiff (PAS) staining (a, b), H&E staining (d, e), and Gomori’s trichrome staining (g, h) were used to measure goblet cell% lining the columnar epithelial cells (c), epithelial layer thickness (f), and subepithelial collagen layer thickness (i), respectively. Green arrows are pointing towards the granulocytes in the peribronchovascular region (d, e) or subepithelial collagen deposition and smooth muscle thickness (g, h). Naïve levels are indicated by the dashed line (c, f, i). Bars represent mean ± SEM,  mice/group. *, #, value < 0.05, as compared with naïve mice or mice challenged with dead conidia, respectively. Scale bars = 200 μm (a, b), 20 μm (d, e), and 100 μm (g, h).
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