Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
Figure 4
Adipogenic differentiation of DMS/PCs. (a) As a negative control, cells cultured in complete medium showed no changes in morphology and were negative for oil red O staining. (b) After induction for 7 days, DMS/PCs became fibroblast-like to oblate and formed many intracellular lipid droplets. Lipid droplets were stained with oil red O (scale bar: 100 μm). (c) Expression of adipocyte-specific genes LPL and PPAR-γ was detected by RT-PCR in the induced group after induction for 14 days. Adipocyte-specific genes were not expressed in the control group. Lane 1: LPL was positive in the inducted group; lane 2: LPL was negative in the control group; lane 3: PPAR-γ was positive in the inducted group; lane 4: PPAR-γ was negative in the control group; lanes 5-6 GAPDH served as the internal control.