5-Lipoxygenase Deficiency Reduces Acetaminophen-Induced Hepatotoxicity and Lethality

Figure 6

Acetaminophen (APAP) induces 5-LO-dependent induction of cytokine production in the liver. WT and 5-LO^−/− mice were treated with APAP (3 g/kg) or saline per oral, and after 12 h liver samples were collected to determine (a) IL-1β, (b) TNF-α, (c) IFN-γ, and (d) IL-10 levels by ELISA. Values are mean ± S.E.M., 𝑛=5, representative of two separate experiments. *𝑃<0.05 compared to saline-treated WT and 5-LO^−/− mice and ^#𝑃<0.05 compared to APAP-treated WT mice. One-way ANOVA was followed by Bonferroni’s multiple comparison test.

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