Figure 2: (a) Experimental protocol for 1st- and 2nd-group animal experiments. MRI images were acquired in time slots I/II for group 1 animals and in time slots I/II/III for group 2 animals; double asterisks (**) indicate group-2 experiments only. (b) FUS-exposed brain of a normal animal with Evans blue extravasations to identify the location of BBB opening. (c) R1 accumulating map showing Gd-DTPA accumulation in the BBB-opening location of a normal animal over time.