CpG ODN and ISCOMATRIX Adjuvant: A Synergistic Adjuvant Combination Inducing Strong T-Cell IFN- Responses
Figure 2
C57Bl/6 mice (/group) were immunized on days 0, 14, and 21 by IM injection of 10 μg OVA either alone (none) or combined with CpG (10 μg), ISCOMATRIX (IMX) (1 μg), or CpG (10 μg) + ISCOMATRIX (1 μg). (a) and (b) each bar represents the group geometric mean (SEM) of the titer for OVA-specific antibodies (anti-OVA IgG) in plasma taken 1 week after 2nd (a) or 3rd (b) immunization. (c) each bar represents the group geometric mean (SEM) of the titer for OVA-specific antibodies (anti-OVA IgG) of IgG1 (open bars) or IgG2c (closed bars) in plasma taken 1 week after final immunization. Titers were defined as the highest plasma dilution resulting in an absorbance value two times that of nonimmune plasma with a cutoff value of 0.05. Representative data from one of five independent experiments is shown in each graph.
