Comparison between the Repression Potency of siRNA Targeting the Coding Region and the 3′-Untranslated Region of mRNA
Figure 2
Fold repression caused by (a) siFLuc-I and (b) siFLuc-II on the reporter vectors pFluc and pRLuc-f. For transfection, the H1299 cells were grown in 6 cm Petri dishes at 70% confluence. One microgram (1 μg) pSEAP2-Control, 1 μg pFLuc or pRLuc-f, and siNC or siFLuc (20 nM final concentration) were mixed and transfected into the cells (see Section 2 for details). Measurement of fold repression is mentioned in Section 2. The data presented are the mean ± SD of triplicates. values are also indicated.
