BioMed Research International / 2013 / Article / Fig 3

Research Article

Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion

Figure 3

Alkaline phosphatase assay. (a) Alkaline phosphatase (ALP) enzyme activity in HOS cells seeded onto the Ln2-P3-treated titanium (Ti) discs was significantly increased as compared with that of the uncoated and SP-coated discs after 1 day. Note that the enzyme activity on the SP-coated surface was significantly lower at day 1 than that on the commercially pure (c.p.) Ti surface with no coating. (b) No significant differences in the ALP activity were observed after 3 days in culture. Data are expressed as the mean ± standard deviation (n = 3). Ti, Ti + SP, and Ti + Ln2-P3 on the graphs mean the uncoated, SP-coated, and Ln2-P3-coated c.p. Ti surfaces, respectively.