Thermal Stability of Glucokinases in Thermoanaerobacter tengcongensis
Figure 4
In vivo (a) and in vitro (b) analysis of interactions between GLKs and HSP60. (a) Co-IP analyses of the interactions between GLKs and HSP60. Anti-HSP60 antibody was used as the bait. After incubation with the native protein lysate either from T. tengcongensis cells cultured at 75°C or 80°C, the precipitated complexes were identified by Western blot with specific anti-ATP-GLK or anti-ADP-GLK antibody. (b) In vitro protein interaction assay of GLKs and HSP60. Recombinant HSP60 was incubated with recombinant ATP-GLK or ADP-GLK in the binding buffer at different temperatures for 30 min. The binding complexes were then precipitated using anti-HSP60 antibody and detected with specific anti-His6 antibody.
