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BioMed Research International
Volume 2013, Article ID 659183, 9 pages
Research Article

Antimicrobial Activity, Growth Inhibition of Human Tumour Cell Lines, and Phytochemical Characterization of the Hydromethanolic Extract Obtained from Sapindus saponaria L. Aerial Parts

1Pharmacognosy Department, National Research Centre, Dokki, Giza 12311, Egypt
2Institute for Biological Research “Siniša Stanković”, University of Belgrade, Boulevard, Despota Stefana 142, 11000 Belgrade, Serbia
3Montain Research Center (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Apartado 1172, 5301-855 Bragança, Portugal
4Centre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal

Received 26 April 2013; Revised 7 October 2013; Accepted 9 October 2013

Academic Editor: Ziad Daoud

Copyright © 2013 Khaled N. Rashed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The hydromethanolic extract of Sapindus saponaria L. aerial parts was investigated for antimicrobial activity (against several Gram-positive and Gram-negative bacteria and fungi) and capacity to inhibit the growth of different human tumor cell lines as also nontumor liver cells. The evaluated extract was further characterized in terms of phytochemicals using UV, 1H-NMR, 13C-NMR, and MS spectroscopic tools. The extract has shown a significant antimicrobial activity on all tested bacterial and fungal species. The best activity was achieved against Bacillus cereus and Staphylococcus aureus among bacteria and against all three Penicillium species tested. It also revealed cytotoxicity against human colon (HCT-15), cervical (HeLa), breast (MCF-7), and lung (NCI-H460) carcinoma cell lines, with HeLa being the most susceptible tumor cell line. The extract was not toxic for nontumor liver cells. Chromatographic separation of the extract resulted in the isolation and identification of stigmasterol, oleanolic acid, luteolin, luteolin 8- - -glucoside (orientin), luteolin 6- - -glucoside (isoorientin), luteolin 7- - -glucuronide, and rutin. The results of the present findings may be useful for the discovery of novel antitumor and antimicrobial agents from plant origin.