Combined Treatment with Low Concentrations of Decitabine and SAHA Causes Cell Death in Leukemic Cell Lines but Not in Normal Peripheral Blood Lymphocytes
Figure 2
Apoptotic features induced by DAC and/or SAHA treatment: appropriate fraction of control cells (empty) or cells treated for 48 h by 1 μM DAC (vertically shaded), 1 μM SAHA (horizontally shaded), or DAC + SAHA combination (filled) measured by flow cytometry. (a) Annexin V-FITC/PI staining of nonfixed cells: Annexin V+/PI− fraction; effect of 10 μM z-VAD-fmk (dotted) or 20 μM Q-VD-Oph (checked). (b) PI staining of ethanol-fixed cells: fraction of cells in subG1 phase. (c) MitoTracker Red fluorescence: fraction of cells having polarized mitochondrial membrane. Error bars represent ±SEM of at least 3 measurements. Statistical significance degree of difference between treated samples and the corresponding control: (*), (**), and (***). (d) Immunoblots showing caspases-3 and -7 activation after 48 h DAC and/or SAHA treatment. (e) Fragmentation of PARP in CML-T1 and HL-60 cell lines or PBL treated by DAC and/or SAHA for 48 h. Images are representatives of at least three experiments.
