Research Article

Combined Treatment with Low Concentrations of Decitabine and SAHA Causes Cell Death in Leukemic Cell Lines but Not in Normal Peripheral Blood Lymphocytes

Figure 5

Production of reactive oxygen species. Control cells (empty) and cells treated for 48 h by 1 μM DAC (vertically shaded), 1 μM SAHA (horizontally shaded), or DAC + SAHA combination (filled) were stained by H2DCFDA, and the mean value of flow-cytometer-detected fluorescence intensity was analyzed. 10 mM NAC was used for DAC + SAHA-induced ROS generation reduction (checked). Error bars represent ±SEM of four measurements. Statistical significance degree of difference between treated samples and the corresponding control: (*), (**), and (***).
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