P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods
Figure 3
Microplate reader and flow cytometer fluorescence responses linearity. MCF-7 drug-sensitive parental cells were incubated during 15 minutes in the dark with 0,05; 0,1; 0,25; 0,5; 0,75, or 1 μM of calcein-AM. Cells were analysed by flow cytometry for mean volume-normalized fluorescence (FL1-FC, (a)) and by a microplate reader for cell-normalized well fluorescence (b). Data are presented as mean ± sem with independent assays per data point.
