Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin
Reactivity of CT-IC with purified recombinant CT and LT. 0.1 mL of serial diluted purified recombinant CT (a) or LT (b) was applied to the test strips. After 15 minutes, development of red color at position for test (T) or control (C) lines was monitored. Concentrations of samples applied are indicated on right side of each strip. The “+++”, “++”, “+”, or “−” symbols are placed on the left side of the strips developing “strong,” “medium,” “faint,” or “no” bands at test lines, respectively.