Rapid Identification of Aldose Reductase Inhibitory Compounds from Perilla frutescens

Figure 2

HSCCC separation of the EtOAc soluble fraction from the leaves of P. frutescens. Solvent system: n-hexane-ethyl acetate-methanol-water (1.5 : 5 : 1 : 5, v/v); flow-rate, 5.0 mL/min; revolution speed, 400 rpm; sample size, 4.0 g; injection volume, 50 mL; detection wavelength, 280 nm; stationary phase retention, 56%. Peaks I, II, III, and IV in the HSCCC chromatogram correspond to compounds 3, 1, 2, and 4, respectively.