Rapid Identification of Aldose Reductase Inhibitory Compounds from Perilla frutescens

Figure 3

HSCCC chromatogram of P. frutescens after HSCCC separation. Solvent system: n-hexane-ethyl acetate-methanol-water (3 : 7 : 5 : 5, v/v); flow rate, 5.0 mL/min; revolution speed, 400 rpm; sample size, 1.2 g; injection volume, 50 mL; detection wavelength, 280 nm; stationary phase retention, 53%. Peaks V and VI in the HSCCC chromatogram correspond to compounds 6 and 5, respectively.