Rapid Identification of Aldose Reductase Inhibitory Compounds from Perilla frutescens

Figure 4

HPLC analysis of compounds 1–6 separated by HSCCC. Conditions: column, Eclipse SB-C18 Rapid Resolution column (150 × 4.6 mm, 5 μm, Agilent); column temperature, 30°C; mobile phase, 0.1% TFA (solvent A) and acetonitrile (solvent B); HPLC analysis, linear gradient from 7.5 to 55% B (0–40 min). Flow rate, 0.7 mL/min; detection, photodiode array detector; injection volume, 10 μL.