Figure 5: Mucosal binding antibody levels. Lung and nasal washes were collected from individual mice before and at day 4 postchallenge with A/Philippines/82 (H3N2) and A/Puerto Rico/8/34 (H1N1) viruses. Lung and nasal washes, with 5-fold dilution and no dilution, respectively, were used to determine IgA and IgG antibody binding to M2e peptide in ELISA. (a) IgA binding in lung washes. (b) IgG binding in lung washes. (c) IgA binding in nasal washes. (d) IgG binding in nasal washes. Each group has 3 mice. The asterisk (*) indicates a significant difference between G2 and G1, G3 and G1, G4 and G1, and G5 and G1. The “+” indicates a significant difference between G3 and G2, G4 and G2, and G5 and G2.