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BioMed Research International
Volume 2013, Article ID 687156, 4 pages
Research Article

Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients

1Department of Microbiology, Medical University of Gdańsk, Do Studzienki 38 Street, 80-227 Gdańsk, Poland
2Department of Pathophysiology, Medical University of Gdańsk, Dębinki 7 Street, 80-211 Gdańsk, Poland
3Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, Dębinki 7 Street, 80-952 Gdańsk, Poland

Received 5 April 2013; Revised 3 June 2013; Accepted 10 June 2013

Academic Editor: Ziad Daoud

Copyright © 2013 T. Jarzembowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal strains isolated from renal transplantation (RTx) recipients than in commensal strains. Additionally, in contrast to commensal strains in isolates from RTx patients, PBP5 gene expression was 17.45% higher in biofilms than in planktonic cells. Detailed comparison also showed that cyclosporine seemed to induce higher expression of PBP5 as compared to tacrolimus.