Figure 2: Analysis of spermatogenesis in unilateral nephrectomized rats treated with cyclosporine, tacrolimus, or sirolimus. Male Sprague-Dawley rats were randomly divided into 5 experiment groups of eight animals each. All animals except for the control group were subjected to tone left unilateral nephrectomy. After recovery from surgery, animals received daily oral gavage of either drinking water or one of the drugs diluted in water for 8 weeks. The testes were harvested at the end of the study, and sperm suspension was prepared from the testis sample for examination under light microscope. UN, unilateral nephrectomy; CsA, cyclosporine; FK506, tacrolimus; Rapa, sirolimus. compared with UN group; compared with UN+FK506 group; compared with UN+Rapa group.