N2 Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
Figure 8
N2 gas plasma treatment causes damage to viral genomic RNA. An aliquot of influenza A virus (A/PR/8/34) infected allantoic fluid was dropped onto a cover glass and air-dried. The dried spot was then treated with N2 gas plasma (1.5 kpps) for the indicated times. The viral genomic RNA of the influenza virus was subsequently extracted and subjected to reverse transcription in order to generate complementary DNA (cDNA). Damage to the genomic RNA of influenza virus induced by the N2 gas plasma treatment was analyzed using the polymerase chain reaction (PCR). Namely, PCR was performed using cDNAs as template and specific sets of primers for matrix protein (M1), nonstructural protein (NS), hemagglutinin (HA), and neuraminidase (NA) of influenza virus. DNA size marker (100 bp DNA ladder Dye plus, Takara Bio Inc.) was run on the left hand lane of the gel.
