Overexpression of RKIP Inhibits Cell Invasion in Glioma Cell Lines through Upregulation of miR-98
Figure 4
Force expression of RKIP enhances miR-98 expression and inhibits HMGA2 expression. U251 or U87 cells were stably infected with vector control and RKIP ORF clone. ((a), (b)) Quantitative RT-PCR quantification of RKIP mRNA or miR-98 expression compared to U251 or U87 cells transfected with vector control. (c) Western blot of RKIP protein expression. Expression of RKIP was forced, and expression of HMGA2 was inhibited by overexpression of RKIP, compared to U251 or U87 cells expressing vector. ((d), (e)) Band intensities were quantitated by Image-Pro Plus. The intensities of the bands corresponding to RKIP and HMGA2 were compared to those corresponding to β-actin and control untransfected U251 or U87. The figure is representative of three experiments with similar results.
