Overexpression of RKIP Inhibits Cell Invasion in Glioma Cell Lines through Upregulation of miR-98
Figure 5
Overexpression of miR-98 enhances the effects of RKIP on miR-98 expression and HMGA2 expression. U251 or U87 cell line was cotransfected with RKIP ORF clone and miR-98 mimics. After 48 h, the level of miR-98 or HMGA2 protein was analyzed by quantitative RT-PCR or western blot. (a) Overexpression of miR-98 increased miR-98 expression. (b) Overexpression of miR-98 enhances the effects of RKIP on miR-98 expression. (c) Overexpression of miR-98 increased the effects of RKIP on HMGA2 protein expression. ((d), (e)) Band intensities were quantitated by Image-Pro Plus. The intensities of the bands corresponding to HMGA2 were compared to those corresponding to β-actin and control cotransfected with miR-SCR and pcDNA3.1 vector. The figure is representative of three experiments with similar results.