High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis

<table>Cardiomyocyte phenotype in HDS. (a) Relative mRNA expression of four cardiac specific genes in early (1 week) and late (4–6 weeks) HDS cultured in Defined medium. (b) Histology of the HDS stained for HE at 1 week. (c) Staining with antibody against <i>α</i>-sarcomeric actin (red) expressed by cardiomyocytes at 1 week and (d) at 9 weeks. A larger magnification is shown to better visualize the staining. (e) Two cardiomyocytes keeping their intense expression of <i>α</i>-sarcomeric actin at 6 weeks. (f) Staining with antibody against TroponinT2 (red) at 1 week and (g) at 6 weeks. DAPI (blue) was used to counterstain nuclei.</table>

BioMed Research International

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Figure 5

Figure 5: High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis 

Figure 5 | High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis 