Research Article

Is the Second Sodium Pump Electrogenic?

Table 1

ATPase activities of microsomal fraction from MDCK cells.

ConditionsMg2+Mg2+ + Na+Mg2+ + Na+ + K+

Control49.25 ± 1.6593.29 ± 2.42183.88 ± 10.31
Ouabain49.52 ± 2.6490.73 ± 3.3395.76 ± 4.20
Ouabain + furosemide52.94 ± 3.0653.26 ± 3.3055.54 ± 4.12

Results are expressed in nmoles Pi Lib./min*mg of protein and represent mean ± SEM of seven independent preparations.