Figure 5: Effect of water extract of bayberry on ROS production in acrylamide-treated Caco-2 cells. (a) After treatment with 5 mM acrylamide in the presence or absence of myricitrin for 0–48 h, Caco-2 cells were incubated with 10 μM DCFH-DA for 30 min and then immediately subjected to fluorescence microscope analysis. (b) The quantitative data of panel (a) and results were expressed as mean DCF fluorescence intensity (means ± SD of three independent experiments). *P value represents significant difference between conditions, where . AA: acrylamide. AA5: 5 mM acrylamide; M5: 5 μg/mL myricitrin; M10: 10 μg/mL myricitrin.