Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs
Figure 3
Verification of pGC-E1-hHGF expression in bacterial clones via PCR. Lane 1: negative control (ddH2O); lane 2: negative control (pGC-E1 empty vector); lane 3: positive control (pcDNA3.0-hHGF); lane 4: marker (5 kb, 3 kb, 2 kb, 1.5 kb, 1 kb, 750 bp, 500 bp, and 250 bp); lanes 5–10: six pGC-E1-hHGF-transformed clones. Clones 7, 8, and 10 tested positive.
