αVβ5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors

Figure 1

Integrin blocking effects on hESCs attachment. hESCs cultured in 2% O₂ or 21% O₂ and preincubated with anti- (a) α6, (b) αE, (c) αV, (d) β5, (e) αVβ5, and (f) CD44 antibodies. (𝑛=6); *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001. 𝑦-axis indicates % of input cell attachment 24 hours after antibody blocking. Black and open bars indicate % of cell attachment in 2% O₂ and 21% O₂ after antibody blocking treatment, respectively. Error bars indicate standard deviations (SD). (g) Cell attachment data normalised to unblocked controls. Asterisks indicate significant differences to unblocked controls. Values indicate mean percentage of cell attachment (𝑛=6); *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001.

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