Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages

Figure 2

Effects of low pH stress on LTA-stimulated cytokine production in rat aMØs. Isolated aMØs were stimulated in vitro with either saline (control), LTA alone (30 mg/mL for 24 hrs), or LTA in combination with a preceding transient low pH stress injury (pH 1.75 for 1 min, Low pH + LTA). Cytokines produced were assessed by ELISA (CINC-1, MIP-2, and MCP-1) or cytotoxicity bioassay (TNF-𝛼). ^∗𝑃<0.05 LTA versus control, ^#𝑃<0.05 LTA versus Low pH + LTA.

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