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BioMed Research International
Volume 2013 (2013), Article ID 748592, 6 pages
Research Article

Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes

1Parasitology Department, Razi Vaccine and Serum Research Institute, P.O. Box 31975-148, Karaj, Alborz, Iran
2Parasitology and Entomology Department, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Iran

Received 9 April 2013; Accepted 27 June 2013

Academic Editor: Terry K. Smith

Copyright © 2013 Vahid Nasiri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The protozoan parasites of the genus Leishmania are the causative agents of different clinical diseases. Fetal calf serum (FCS) is the main part and the most expensive ingredient of the Leishmania culture media. Here, the efficacies of different concentrations (1%, 2.5%, 5%, and 10%) of the filtered and autoclaved sheep and mouse urine were evaluated as a growth stimulator in Leishmania culture procedure. The results indicated that culture media enriched with the filtered sheep and mouse urine supported the growth of the parasites and can be used for cultivation of Leishmania parasites. In conclusion, this study has demonstrated an alternative low-cost medium that could be used in cultivation process of Leishmania major promastigotes.