BioMed Research International / 2013 / Article / Fig 3

Review Article

Potential Use of Atlantic Cod Trypsin in Biomedicine

Figure 3

The ability of different proteases to cleave the surface receptors CD4 (a), CD8 (b), CD31 (c), CD11a/CD18 (d), CD102 (e), and CD62L (f) at the surface of freshly isolated murine T cells [27]. The proteases used are shown on the left side of the Figures (a)–(f). The proteases were used at a final concentration of 2 μg/mL (blue bars) and 20 μg/mL (red bars) for 2 h at 37°C. The bars represent the relative amount of undegraded surface receptors after digestion by the proteases tested, that is, the smaller the bars the higher the proteolytic efficacy. Each assay was carried out in duplicate or triplicate and the cells were T lymphocytes of murine and human origin. Each column represents average numbers. Standard deviation ranged from 0.01 to 0.1.

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