High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli

Figure 1

Schematic representation of the vector constructions and the expression of the N-terminal fusion and C-terminal fusion proteins. In the C-terminal fusion protein, intein2-CBD was fused to the C-terminus of OG2, allowing the cleavage of OG2 from OG2-intein2 using DTT. In the N-terminal fusion protein, CBD-intein1 was fused to the N-terminus of OG2, allowing the cleavage of OG2 from intein1-OG2 through a pH and temperature shift.