High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli
Figure 4
Large-scale expression, purification, and activity of OG2. (a) Purification (10 mL chitin) and on-column cleavage of OG2-intein2. lane M, SeeBlue Plus2 Pre-Stained Standard (kDa); lane SP, soluble protein; lane FT: flowthrough; lane F, proteins eluted from the chitin column with 2% SDS; lane P, released OG2. (b) Comparison of the antimicrobial activities of chemically synthesized OG2 (left) and expressed OG2 (right). For expressed OG2, both the halo with Oxford cup (top) and the halo without the cup (bottom) are shown.
