High Glucose-Induced Oxidative Stress Increases the Copy Number of Mitochondrial DNA in Human Mesangial Cells
Figure 1
Effect of high glucose on mtDNA copy number. Synchronized quiescent mesangial cells were incubated with 5 mM glucose, 25 mM glucose, and 5 mM glucose plus 20 mM mannitol for 24 h. The copy numbers of ND2 and CYTB per B2M were determined by real-time PCR. Values represent mean ± SD of three independent experiments. compared to 5 mM glucose and to 5 mM glucose plus 20 mM mannitol.
