High Glucose-Induced Oxidative Stress Increases the Copy Number of Mitochondrial DNA in Human Mesangial Cells
Figure 4
Inhibition of mitochondrial superoxide by MnTBAP in human mesangial cells. Synchronized quiescent mesangial cells grown on glass coverslips were incubated for 24 h with 25 mM glucose with and without MnTBAP (200 μM) or 5 mM glucose, loaded with MitoSOX (5 μM, 20 min), and examined by confocal microscopy. (a) Representative confocal images of human mesangial cells showing decrease in mitochondrial MitoSOX fluorescence following incubation of the cells with 25 mM glucose and MnTBAP. (b) Quantitative analysis of the MitoSOX fluorescence intensity per square millimeter cell area in human mesangial cells. Values represent mean ± SD of three independent experiments. compared to 25 mM glucose without MnTBAP.
