Hepatoprotective and Antioxidant Effects of Saponarin, Isolated from Gypsophila trichotoma Wend. on Paracetamol-Induced Liver Damage in Rats

Table 3

Cytochrome P450, EMND, and AH activities measured in rat liver microsomes.

Group	EMND activity nmol/mg protein	AH activity nmol/mg protein	Cytochrome P450 nmol/mg protein
Control	0.486 ± 0.032	0.075 ± 0.003	0.325 ± 0.04
Paracetamol	0.467 ± 0.02	0.078 ± 0.005	0.345 ± 0.04
Saponarin	0.371 ± 0.011*	0.047 ± 0.004*	0.244 ± 0.05*
PC + SP	0.375 ± 0.006^*+	0.048 ± 0.005^*+	0.236 ± 0.03^*+
Silymarin	0.340 ± 0.013*	0.051 ± 0.005*	0.255 ± 0.03*
PC + SL	0.346 ± 0.02^*+	0.048 ± 0.004^*+	0.262 ± 0.02^*+

Treatment: paracetamol administered at a dose of 600 mg/kg i.p alone and two hours after the last administration of the hepatoprotective agents (SP + PC and SL + PC groups); saponarin (SP) (80 mg/kg bw/day) and silymarin (SL) (100 mg/kg bw/day) were administered p.o. for 7 days. Data are expressed as mean ± SEM of six rats. *Significant difference from control values (Mann-Whitney test, 𝑃<0.05). ⁺Significant difference from paracetamol-treated group (Mann-Whitney test, 𝑃<0.05).