Exploring the Possible Role of Lysine Acetylation on Entamoeba histolytica Virulence: A Focus on the Dynamics of the Actin Cytoskeleton
Figure 1
Aspirin acetylates actin in E. histolytica without affecting the growth of parasites. (a) Growth curves for untreated and Aspirin (1 mM), CD (10 μM), or DMSO-treated E. histolytica. (b) Trophozoites were treated with 1 mM Aspirin, 50 μm Indo, or 50 μm NS-398 for 2 h, lysates prepared and immunoblotted with antiacetyl-lysine antibody (1 : 200). (c) Confocal microscopy analysis of trophozoites treated or not with 1 mM Aspirin. Actin was stained with rhodamine-phalloidin (red) (1 : 25), and acetylated proteins (green) were detected with antiacetyl-lysine antibody (1 : 50) and FITC-labeled goat anti-rabbit IgG (1 : 100). (d) Transmission electron micrographs show a section of trophozoites treated or not with 1 mM Aspirin, where the association between gold-labeled acetyl-lysine antibody (large particles) and actin (small particles) (arrows) is clear. (e) Detection by western blot of the acetylated level of immunoprecipitated proteins from treated (Asp) and nontreated (Ctl) trophozoites with the antiacetyl-lysine antibody (1 : 200).
