TY - JOUR
A2 - Kesari, Akanchha
AU - Tuononen, Katja
AU - Sarhadi, Virinder Kaur
AU - Wirtanen, Aino
AU - Rönty, Mikko
AU - Salmenkivi, Kaisa
AU - Knuuttila, Aija
AU - Remes, Satu
AU - Telaranta-Keerie, Aino I.
AU - Bloor, Stuart
AU - Ellonen, Pekka
AU - Knuutila, Sakari
PY - 2013
DA - 2013/01/20
TI - Targeted Resequencing Reveals ALK Fusions in Non-Small Cell Lung Carcinomas Detected by FISH, Immunohistochemistry, and Real-Time RT-PCR: A Comparison of Four Methods
SP - 757490
VL - 2013
AB - Anaplastic lymphoma receptor tyrosine kinase (ALK) gene rearrangements occur in a subgroup of non-small cell lung carcinomas (NSCLCs). The identification of these rearrangements is important for guiding treatment decisions. The aim of our study was to screen ALK gene fusions in NSCLCs and to compare the results detected by targeted resequencing with results detected by commonly used methods, including fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and real-time reverse transcription-PCR (RT-PCR). Furthermore, we aimed to ascertain the potential of targeted resequencing in detection of ALK-rearranged lung carcinomas. We assessed ALK fusion status for 95 formalin-fixed paraffin-embedded tumor tissue specimens from 87 patients with NSCLC by FISH and real-time RT-PCR, for 57 specimens from 56 patients by targeted resequencing, and for 14 specimens from 14 patients by IHC. All methods were performed successfully on formalin-fixed paraffin-embedded tumor tissue material. We detected ALK fusion in 5.7% (5 out of 87) of patients examined. The results obtained from resequencing correlated significantly with those from FISH, real-time RT-PCR, and IHC. Targeted resequencing proved to be a promising method for ALK gene fusion detection in NSCLC. Means to reduce the material and turnaround time required for analysis are, however, needed.
SN - 2314-6133
UR - https://doi.org/10.1155/2013/757490
DO - 10.1155/2013/757490
JF - BioMed Research International
PB - Hindawi Publishing Corporation
KW -
ER -