Table 1: ALK positive cases detected by FISH, IHC, real-time RT-PCR, and targeted resequencing.

Sample IDAgeSexSmokingFISH IHC RT-PCR Targeted resequencing
Fusion type/% of positive cellsVariant typeFusion/break position in ALK

LC146FNonsmokerSingle-signal/70Strongly positiveEML4-ALK variant 1/2/3a/3b
chr2: 29447508–29447608
LC1148MNonsmokerPair-signal/46Strongly positiveEML4-ALK variant 1/2/3a/3b
chr2: 29446498–29446566
LC14*44MNonsmokerPair-signal/74Not studied EML4-ALK variant 1/2/3a/3bchr2: 29446561–29446661
Strongly positive
EML4-ALK variant 1/2/3a/3b
chr2: 29446561–29446661
LC5151MNonsmokerSingle-signal/66Strongly positiveEML4-ALK variant 1/2/3a/3b
chr2: 29446527–29446625
1591563MNonsmokerSingle-signal/40Strongly positiveEML4-ALK variant 1/2/3a/3b
Not studied

*LC14 and LC74 are samples from the same patient but from a different FFPE block.