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BioMed Research International
Volume 2013 (2013), Article ID 761451, 9 pages
Research Article

Differential Influence of Components Resulting from Atmospheric-Pressure Plasma on Integrin Expression of Human HaCaT Keratinocytes

1Institute of Pharmacy, Department of Pharmaceutical Biology, University of Greifswald, Friedrich-Ludwig-Jahn-Straße 17, 17487 Greifswald, Germany
2Leibniz Institute for Plasma Science and Technology e.V. (INP), Campus PlasmaMed/PlasmaVitro, Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany
3ZIK plasmatis, Leibniz Institute for Plasma Sciences and Technology e.V. (INP), Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany

Received 2 April 2013; Accepted 10 June 2013

Academic Editor: Maxim E. Darvin

Copyright © 2013 Beate Haertel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Adequate chronic wound healing is a major problem in medicine. A new solution might be non-thermal atmospheric-pressure plasma effectively inactivating microorganisms and influencing cells in wound healing. Plasma components as, for example, radicals can affect cells differently. HaCaT keratinocytes were treated with Dielectric Barrier Discharge plasma (DBD/air, DBD/argon), ozone or hydrogen peroxide to find the components responsible for changes in integrin expression, intracellular ROS formation or apoptosis induction. Dependent on plasma treatment time reduction of recovered cells was observed with no increase of apoptotic cells, but breakdown of mitochondrial membrane potential. DBD/air plasma increased integrins and intracellular ROS. DBD/argon caused minor changes. About 100 ppm ozone did not influence integrins. Hydrogen peroxide caused similar effects compared to DBD/air plasma. In conclusion, effects depended on working gas and exposure time to plasma. Short treatment cycles did neither change integrins nor induce apoptosis or ROS. Longer treatments changed integrins as important for influencing wound healing. Plasma effects on integrins are rather attributed to induction of other ROS than to generation of ozone. Changes of integrins by plasma may provide new solutions of improving wound healing, however, conditions are needed which allow initiating the relevant influence on integrins without being cytotoxic to cells.